Metabolites are the key nodes of metabolic networks and can function as energy carrier, building blocks for biosynthesis and signalling molecules with regulatory functions. The homeostasis of metabolite pools and the dynamic of metabolite fluxes are essential for intracellular metabolism and also for complex interspecies microbial networking. In this facility, we will provide state of the art metabolomics approaches to the research groups of the CRC, using chromatography and mass spectrometry techniques. We will enable the researchers to perform metabolomics experiments to obtain metabolic profiles, monitor intracellular and extracellular metabolite pool sizes and measure fluxes via incorporation of stable isotopes.